This Research Topic is part of the Plant Viruses series:Volume I: Plant Viruses: Detection Methods, Genetic Diversity and Evolution Volume II: Molecular Plant Virus Epidemiology and its ManagementPlant viruses have been recognized as economically important plant pathogens worldwide, since they can cause serious losses to various food crops. 3 Detection . The techniques are: 1. Transmission by Mechanical Means 4. Therefore, detection methods should be more convenient, effective, specific and permitted the use for detecting plant pathogens (McCartney et al., 2003).
High-throughput and/or high speed microscopy has also been developed to identify and/or isolate viruses. 1, Ho-jong Ju. The only published applications of the MinION to sequencing plant virus genomes involved the detection of maize streak virus, maize yellow mosaic virus and maize totivirus in maize plants … 1 - UF/IFAS. Dry Examination 2. Therefore, the method covers these two viruses as well. The techniques available have evolved significantly in the last few years to achieve rapid and reliable detection of pathogens, extraction of the target from the sample being important for optimising detection. Narayanasamy P (2011) Detection of virus and viroid pathogens in plants. It is often said that molecular methods is the future direction of viral diagnosis. Traditionally cell culture-based virus isolation has been regarded as “gold standard” for the detection and diagnosis of viruses, and it is the technique to which all other test methods have been compared with .
Naidu RA, Hughes Jd’A (2001) Methods for the detection of plant virus diseases. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. The serological methods of diagnosis, detection and identification of viruses in plants play a vital role [1]. International Institute of Tropical Agriculture, Ibadan, pp 233–260 Google Scholar. Biological Methods 3. R. A. Naidua; Plant viruses cause major losses to several agricultural and horticultural crops around the world. The methods for detection and identification of viruses are critical in virus disease management (Aboul-Ata et al., 2011).
However, the sensitivity for bacteria is relatively low (10 5 –10 6 CFU/mL, Table 1 ) making it useful only for the confirmation of plant diseases after visual symptoms appear but not for early detection before disease symptoms occur [ 12 ]. Virus detection is the first step in the quest for plants that are free of targeted viruses. Revision history: Version 6.3, September 2019 PRINCIPLE Detection of infectious tobamoviruses in pepper seed by inoculating leaves of indicator plants Nicotiana tabacum cv. Traditional diagnosis of plant viruses requires bioassay, an indicator plant, determination of host range, symptomatology, virus particle morphology (size and shape), and vector relations [2,3]. Molecular diagnostic procedures have been described in a number of recent books and articles. For plant disease detection, tissue print-ELISA and lateral flow devices that enable detection have been fabricated for on-site detection. For the majority of the virus species that infect berry crops, there are rapid, sensitive laboratory tests available. Proceedings of a conference organized by IITA. Nevertheless, molecular techniques have revolutionized plant virus detection and identification. Insect Transmission 7. Transmission by Vegetative Propagation 3. Soil Transmission 6.
Serological Methods. High-throughput and/or high speed microscopy has also been developed to identify and/or isolate viruses. In: Hughes Jd’A, Odu BO (eds) Plant virology in Sub-Saharan Africa. Joo-jin Jeong. The following points highlight the top four techniques for detection of seed borne viruses. Methods for the detection of plant virus diseases. Technique # 1. Methods based on the detection of viral genome are also commonly known as molecular methods. Biological indexing and serological enzyme-linked immunosorbent assay (ELISA) methods are widely used for diagnosis. Biochemical Method 4. Narayanasamy P (2011) Detection of virus and viroid pathogens in plants. Xanthi NN or Nicotiana glutinosa with pepper seed extract. 1,2 * and Jaejong Noh. Show less . Seed Transmission of Virus 2. Infectious virions cause typical local lesions that demonstrate viability of the virus. glutinosa). Transmission by Fungi 8.